Original Article

Role of oxidative stress in patients with primary open angle glaucoma and senil cataract - Original Article

  • Özcan Ocakoglu
  • Huriye Balci
  • Ahmet Özkök
  • Yildiz Dinçer
  • Eylem Efe Koç
  • Didar Uçar
  • Tülay Akçay

Received Date: 11.02.2008 Accepted Date: 24.05.2008 Turk J Ophthalmol 2008;38(4):292-297

Purpose: Contribution of oxidative stress in various ocular disorders is known.In our study, the role of oxidative stress in patients with primary open angle glaucoma (POAG) and  senile cataract (SC) was investigated. For this purpose, the products of free radical metabolism and activities of antioxidant enzymes in blood plasma and aqueous humor were measured. Material-Methods: In 27 patients with POAG and 25 patients with SC,blood plasma before surgery and aquous humor at the time of surgery were obtained. Malondialdehyde (MDH), reduced glutathion (GSH) and glutathione peroxidase (G-Px) was studied with spectrophotometrically in plasma; 8-hydroxydeoxyguanosine (8 OH-dG) was measured with ELISA method. This values were compared with results of 28 age matched healthy individuals.On the other hand, in aqueous humor, which is obtained from eyes with glaucoma and/or cataract, 8OH-dG levels were measured. In all three groups, age and gender comparison was made with Chi-square test; differences of MDA, GSH, G-Px and 8OH-dG levels were studied with unpaired ttest. Significance level was accepted as p Results: In all study groups no significant difference between age and sex found (p>0.05). In patients with either POAG or SC, the level of GSH and G-Px, which are the indicators of antioxidant potential, was found half and 1/3 of the level of control group, respectively. The levels of MDA and 8OH-dG, which are free radical metabolits, in patients with glaucoma and/or cataract almost twice the level in control group (p0.05). Conclusion: In patients with POAG or SC, plasma levels of potential antioxidant indicators were found lower than control group and free radical metabolism products higher than control group. These results suggests that oxidative stress may be playing a role in pathogenesis of both of these two diseases.

Keywords: Oxidative stres, primary open angle glaucoma, senile cataract, antioxidant potential

Full Text (Turkish)