Original Article

A Study on Feasibilty of Intravitreal Implantation of Bone Marrow-Derived Mesenchymal Stem Cells in the Rabbit Tapetoretinal Degeneration Model - Original Article

  • Gökhan Özge
  • Güngör Sobaci
  • Ferit Avcu
  • Mükerrem Safali
  • Kamuran Pamuk
  • Ali Ugur Ural

Received Date: 24.03.2008 Accepted Date: 02.07.2008 Turk J Ophthalmol 2008;38(4):310-319

Purpose: To investigate feasibility of intravitreal implantation of bone marrow-derived mesenchymal stem cells(MSC) in the rabbit tapetoretinal degeneration model. Methods: The fourth passage MSC harvested from proximal femur of an adult New Zealand albino rabbit, and differentiated from stromal cells under in-vitro conditions, were used at GATA Medical Research Center. Intravenous injection of Sodium iodate, 40 mg/kg, was applied to 6 adult pigmented rabbits (study group). Subsequently, the MSC (2.5x10(5) cells/0.1ml) were injected intravitreally in the right eyes of these animals and 1 additional pigmented rabbit (control); the left eyes were injected BSS. Ophthalmascopic, fluorescein angiographic, OCT, ERG and histopathologic examinations were performed 1, 5, 10, 20, and 30 days after intravitreal injection. Results: On the 1st. day examination, abnormal ERG parameters in the study group, and minimal irregularity and edema of the inner retinal layers and the RPE in both eyes of 1 rabbit examined histopathologically, were observed. On the 5th day, loss of b waves in ERG, retinal thickening in OCT, and irregularity of the inner retinal and photoreceptor layers in histopathologic examination of 1 rabbit in the study group were observed. On the 10th day, hyperfluorescent and hypofluorescent spots in the FA, loss of ERG b wave in the study group, and irregularity of photoreceptor layer with remarkable pigmentary dispersion extending from the RPE to the Choroid, more evident in the left eye of histopathologically examined one were noted. On the 20th day; apparent pigmentary degeneration in both eyes associated with increased scotopic-ERG amplitudes, more evident in the right eyes, were found. Higher b-wave amplitude in the right eye maintained also on the 30th day examination. No  linical or histological inflammation sign was observed in the right eye of the control rabbit. Conclusions: Intravitreal allogenic stem cell implantation in eyes with tapetoretinal degeneration seems to be feasible method.

Keywords: intravitreal implantation, mesenchymal stem cell, rabbit, tapetoretinal degeneration

Full Text (Turkish)